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Assay Diluents

These assay diluents equalize sample matrix differences and reduce nonspecific interactions between the sample matrix proteins and the plate surface.

BulletReduce matrix effects with assay diluents -

Assay diluents are proprietary buffer formulations designed to equalize any differences between the sample matrix (serum, plasma, urine, cell culture fluid, etc.) and the diluent used to generate the standard curve. These diluents also reduce nonspecific interactions between the sample matrix proteins and the plate surface which translates into lower background noise.

 

Assay diluents are pipetted directly onto the plate just prior to adding the samples, and may be used with neat or diluted samples, depending on the characteristics of the sample matrix and the target range of the assay. Since different sample matrices have unique characteristics when run in an ELISA, they must be matched with specific assay diluents. Use of an assay diluent reduces the effects of the sample matrix and variation among samples, without pre-dilution of the samples.

 

We are pleased to offer 4 proprietary assay diluent formulations for use with sandwich and antigen-down ELISAs.

 

BulletGeneral Assay Diluent -
Suitable for all ELISAs; for serum and plasma samples tested in all antibody sandwich ELISAs.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-620 General Assay Diluent 100 ml
$ Call us
REA-621 General Assay Diluent 500 ml
$ Call us
REA-622 General Assay Diluent 1 liter
$ Call us
REA-671 General Assay Diluent 10 liters
$ Call us
 
BulletIgM Reducing Assay Diluent -
All ELISAs; for serum and plasma samples tested in all antibody sandwich ELISAs; to inhibit complement and thrombin activity; to reduce IgM-mediated conjugate bridging interference .
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-623 IgM Reducing Assay Diluent 100 ml
$ Call us
REA-624 IgM Reducing Assay Diluent 500 ml
$ Call us
REA-625 IgM Reducing Assay Diluent 1 liter
$ Call us
REA-672 IgM Reducing Assay Diluent 10 liters
$ Call us
 
BulletNeptune Assay Diluent -
Antigen Down and Sandwich ELISAs; for serum and plasma samples tested in all antigen-down ELISAs; for human, porcine, and bovine serum samples; to inhibit complement and thrombin activity.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-626 Neptune Assay Diluent 100 ml
$ Call us
REA-627 Neptune Assay Diluent 500 ml
$ Call us
REA-628 Neptune Assay Diluent 1 liter
$ Call us
REA-673 Neptune Assay Diluent 10 liters
$ Call us
 
BulletAntigen-Down Assay Diluent -
Antigen Down and Sandwich ELISAs; for serum and plasma samples tested in all antigen-down ELISAs; to eliminate clotting of plasma samples in the ELISA plate well.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-629 Antigen-Down Assay Diluent 100 ml
$ Call us
REA-630 Antigen-Down Assay Diluent 500 ml
$ Call us
REA-631 Antigen-Down Assay Diluent 1 liter
$ Call us
REA-674 Antigen-Down Assay Diluent 10 liters
$ Call us
 
BulletOptimization Pack -
includes 1 bottle of each assay diluent (100 mL each). We can give you a good idea of which assay diluent to try, but sometimes, it's just best to try each one in your assay system.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-958 Assay DiluentsOptimization Pack 4 x100 mls 4x100mls
$ Call us