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Blocking Buffers

These buffers can reduce background, stabilize antigenic domains, and increase assay sensitivity and reproducibility . They can also increase the efficiency and extend the shelf-life of coated plates.

BulletBlocking Buffers -

Once the antibody or protein antigens have been properly adsorbed onto the plate the next critical step in creating a reliable assay is the blocking of the plate. Blocking is done to protect the coated protein from harsh external conditions while masking any uncoated regions on the plate. As the blocking process has a profound effect on both the specific and nonspecific signals generated in the assay, the proper selection of block buffer is a key element in the preparation of a functional ELISA. The proper block buffer can increase sensitivity, reduce nonspecific binding, and lengthen the shelf-life of the coated plate.

 

High performance blocking buffers can:

Increase the sensitivity of your assay. This is done by stabilizing the antigenic and functional regions of the adsorbed protein.

 

Reduce background noise. By inhibiting nonspecific binding to uncoated regions of the plate and "sticky" sections of the adsorbed proteins.

 

Increase efficiency and extend the shelf-life of the coated plates. With a longer shelf-life, plates may be prepared in large batches to be used over time.  Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency.

 

Increase assay reproducibility. By using consistent reagents from a reliable source, your ELISAs may become more reproducible.

 

Conserve valuable reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.

 

Because ELISAs may be configured in several ways, each with unique blocking requirements, We offer 3 proprietary block buffer formulations for use with sandwich and antigen-down ELISAs.

 

BulletGeneral Low Level Blocker -

A buffer based on mammalian proteins from BSA. Use this blocker for:

Sandwich and antigen-down ELISAs requiring low to average blocking strength.

CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-632 General Low level Blocking Buffer 100 ml
$ Call us
REA-633 General Low level Blocking Buffer 500 ml
$ Call us
REA-640 General Low level Blocking Buffer 1 liter
$ Call us
REA-659 General Low level Blocking Buffer 10 liters
$ Call us
 
BulletNeptune Blocking Buffer -

Based on nonmammalian proteins from fish, for extra blocking strength. Use this blocker for:

Antigen-down ELISAs, Sandwich ELISAs with high backgrounds and ELISAs testing human and other mammalian samples.

CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-62 Neptune Blocking Buffer 100 ml
$ Call us
REA-63 Neptune Blocking Buffer 500 ml
$ Call us
REA-64 Neptune Blocking Buffer 1 liter
$ Call us
REA-660 Neptune Blocking Buffer 10 liters
$ Call us
 
BulletSyn Block -

Based on small synthetic blocking molecules, SynBlock is designed for ELISAs that require extra blocking strength. Use this blocker for:

Sandwich ELISAs requiring extra blocking, Antigen-down ELISAs requiring extra blocking and ELISAs with high nonspecific binding of enzyme-labeled conjugates.

CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-641 SynBlock Blocking Buffer 100 ml
$ Call us
REA-642 SynBlock Blocking Buffer 500 ml
$ Call us
REA-643 SynBlock Blocking Buffer 1 liter
$ Call us
REA-661 SynBlock Blocking Buffer 10 liters
$ Call us
 
BulletOptimization Pack -
Includes 1 bottle of each block buffer (100 mL each). We can give you a good idea of which block buffer to try, but sometimes, it's just best to try each one in your assay system.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-957 Blocking Buffers Optimization Pack 3 x 100ml
$ Call us