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Conjuage Diluents

These conjugate stabilizer diluents are designed to prolong the shelf-life of HRP-conjugated proteins and can be used to reconstitute lyophilized conjugates and to dilute concentrated conjugates into the useful range of the assay.

Bullet Stabilize your conjugates with these diluents -

Due to denaturization of the HRP-IgG protein complex and destabilization of the catalytic region of the HRP; HRP-conjugated antibodies can lose a significant level of activity within a short time after rehydration. These unique stabilizing diluents contain a proprietary protein additive to stabilize conjugated proteins, and magnesium and calcium salts to stabilize the catalytic site of the HRP porphyrin ring structure.

 

In addition, these buffer formulations provide the optimal pH and ionic conditions to maintain the native tertiary structure of the conjugate proteins during storage, and antimicrobial agents to retard bacterial growth. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.

 

These reagents were also designed to minimize nonspecific binding interactions between the conjugate and the plate well surface, and to confer additional stability to the overall conjugate structure. Selected buffers contain a proprietary detergent to further reduce nonspecific binding of the conjugate to the plate surface while maintaining HRP activity.

 

Bullet Monoclonal / Polyclonal Conjugate Diluent & Stabilizer -
A unique formulation specifically designed for use in mouse monoclonal capture and polyclonal-HRP sandwich ELISA formats. This diluent contains mouse proteins which suppress nonspecific binding of the polyclonal-HRP conjugate to the mouse immunoglobulins adsorbed onto the plate. These protein additives also provide additional structural stability to the conjugate protein complex. Other additives contained in this stabilizer serve to extend the useful titer of the conjugate by stabilizing both the immunoglobulin and HRP components of the conjugate
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-6100 Mono/Poly Conjugate Diluent 5X 1 liter
$ Call us
REA-6101 Mono/Poly Conjugate Diluent 5X 10 liters
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REA-6168 Mono/Poly Conjugate Diluent 5X 25 ml
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REA-698 Mono/Poly Conjugate Diluent 5X 100 ml
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REA-699 Mono/Poly Conjugate Diluent 5X 500 ml
$ Call us
 
Bullet Antigen-Down Conjugate Diluent & Stabilizer -
A unique formulation specifically designed for use with anti-IgG-HRP conjugates. This diluent contains proprietary non-mammalian protein additives which will not interfere with anti-IgG-HRP conjugates used in antigen-down and sandwich ELISA formats (Does not contain BSA). These additives also provide additional structural stability to the IgG-HRP conjugate protein complex, and will inhibit conjugate binding to nonspecific serum proteins bound to the plate, thus reducing conjugate bridging on the plate surface and lowering background noise.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-6102 Antigen-Down Conjugate diluent 5X 100 ml
$ Call us
REA-6103 Antigen-Down Conjugate diluent 5X 500 ml
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REA-6104 Antigen-Down Conjugate diluent 5X 1 liter
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REA-6105 Antigen-Down Conjugate diluent 5X 10 liters
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REA-6169 Antigen-Down Conjugate diluent 5x 25 ml
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Bullet Polyclonal / Polyclonal Conjugate Diluent & Stabilizer (CD3) -
a unique formulation specifically designed for use in polyclonal capture and polyclonal-HRP sandwich ELISA formats. Proprietary additives were specifically selected to provide optimal assay performance when using rabbit, goat, or rabbit and goat polyclonal antibodies in a sandwich ELISA format. This product contains a uniquely processed form of rabbit and goat IgG to suppress nonspecific binding of the polyclonal-HRP conjugates to the plate surface. To reduce nonspecific binding interactions, a detergent is also included
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-6106 Poly/Poly conjugate diluent 5X 100 ml
$ Call us
REA-6107 Poly/Poly conjugate diluent 5X 500 ml
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REA-6108 Poly/Poly conjugate diluent 5X 1 liter
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REA-6109 Poly/Poly conjugate diluent 5X 10 liters
$ Call us
REA-6170 Poly/Poly conjugate diluent 5X 25 ml
$ Call us
 
BulletMonoclonal / Goat-Polyclonal Conjugate Diluent & Stabilizer -
a unique formulation specifically designed for use in monoclonal capture and goat polyclonal-HRP sandwich ELISA formats. Additives were carefully selected to specifically address the stability and nonspecific binding issues associated with this type of ELISA format. Goat proteins are included to reduce any nonspecific interactions between the monoclonal capture and goat polyclonal-HRP antibodies. This product also contains mouse IgG to specifically suppress nonspecific bridging of the goat polyclonal-HRP conjugate with the mouse capture IgG on the plate surface. To further reduce nonspecific binding of the conjugate to the plate surface, a detergent is also included.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-6110 Mono/Goat-Poly conjugate diluent 5x 100 ml
$ Call us
REA-6111 Mono/Goat-Poly conjugate diluent 5x 500 ml
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REA-6112 Mono/Goat-Poly conjugate diluent 5x 1 liter
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REA-6113 Mono/Goat-Poly conjugate diluent 5x 10 liters
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REA-6171 Mono/Goat-Poly conjugate diluent 5x 25 ml
$ Call us
 
BulletMonoclonal / Rabbit-Polyclonal Conjugate Diluent & Stabilizer -
a unique formulation specifically designed for use in monoclonal capture and rabbit polyclonal-HRP sandwich ELISA formats. Rabbit proteins are included to reduce any nonspecific interactions between the monoclonal capture and rabbit polyclonal-HRP antibodies. This product also contains mouse IgG to specifically suppress nonspecific bridging of the rabbit polyclonal-HRP conjugate with the mouse capture IgG on the plate surface.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-6114 Mono/Rabbit-Poly conjugate diluent 5x 100 ml
$ Call us
REA-6115 Mono/Rabbit-Poly conjugate diluent 5x 500 ml
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REA-6116 Mono/Rabbit-Poly conjugate diluent 5x 1 liter
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REA-6117 Mono/Rabbit-Poly conjugate diluent 5x 10 liters
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REA-6172 Mono/Rabbit-Poly conjugate diluent 5x 25 ml
$ Call us
 
BulletConjugate Stock Stabilizing Reagent -
contains a BSA-based mammalian protein additive to stabilize conjugated proteins, and magnesium and calcium salts to stabilize the catalytic site of the HRP porphyrin ring structure. When diluted to 1x, this is an excellent HRP conjugate diluent and stabilizer base, to which additional  components can be added to for use in your specific assay.
CATALOG # DESCRIPTION QTY PRICE
PURCHASE
REA-6173 Conjugate Stock Stabilizing Reagent 5x 25 ml
$ Call us
REA-667 Conjugate Stock Stabilizing Reagent 5x 100 ml
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REA-668 Conjugate Stock Stabilizing Reagent 5x 500 ml
$ Call us
REA-669 Conjugate Stock Stabilizing Reagent 5x 1 liter
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REA-670 Conjugate Stock Stabilizing Reagent 5x 10 liters
$ Call us