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Washing Buffer

A system of buffers for efficient and reliable coating of plates with antibodies or antigens.

BulletWashing Buffer -

ELISA Wash Buffer is used to rinse microtiter plates during the coating process and between reagent addition steps of an ELISA, using an optimal formulation of pH stabilizers, salts, and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background noise and increasing the specific signal. ELISA Wash Buffer is compatible with all routinely used conjugate components such as HRP, AP, and avidin, among others.


ELISA Wash Buffer is supplied as a 10x concentrate to conserve valuable storage space. Simply add 9 parts deionized water to 1 part ELISA Wash, mix, and it’s ready to use. No pH adjustment is required. ELISA Wash contains a non-azide non-mercury preservative that will not interfere with antibody-antigen binding interactions. After dilution, ELISA Wash will suppress bacterial growth for up to 6 months at room temperature and 12 months at 2°-8°C. The concentrated buffer will suppress bacterial growth for at least 18 months at 2°-8°C.


REA-6251 Wash Buffer 10X 25 ml
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REA-650 Wash Buffer 10X 100 ml
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REA-651 Wash Buffer 10X 500 ml
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REA-652 Wash Buffer 10X 1 liter
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REA-676 Wash Buffer 10X 10 liters
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